A Secret Weapon For thr77
A Secret Weapon For thr77
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not nonetheless out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This tutorial outlines even more possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary which is degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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